| Gene | Stxbp5l |
| Chromosome | 16 |
| Deletion Start | 37,383,694 |
| Deletion End | 37,345,076 |
| Deletion Size | 38,619 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 37,383,648 | 37,383,669 | TGGATGGCTTAACTGCCTCTTC |
| TUP | 37,383,626 | 37,383,647 | CCCTGGCAGTGGTAGCAGCAGT |
| TUR | 37,383,584 | 37,383,603 | TGGATGGACAGAGCCACTTC |
| TDF | 37,345,142 | 37,345,163 | TCGGCATGGGTTTCCTTATCAG |
| TDP | 37,345,115 | 37,345,141 | CCCACAGCATTGGCCTTTGATCCAGTC |
| TDR | 37,345,080 | 37,345,102 | GCACCTGTTCTTGTTCCAATAGC |
PCR Primers
| Primer | Sequence |
| SU | GCTCTTGGAGTTGTGTTCTC |
| SD | TACTGCCGTAAGCAAACAATA |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15817A-C11 | VGB6 | C57BL/6NTac | |
| 15817A-D6 | VGB6 | C57BL/6NTac | |
