| Gene | Tomm70a |
| Chromosome | 16 |
| Deletion Start | 57,152,777 |
| Deletion End | 57,121,865 |
| Deletion Size | 30,913 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 57,122,284 | 57,122,301 | CGGAAACTGCCGAGTGAC |
| TUP | 57,122,305 | 57,122,333 | AGAGTCGTTTCTGGTTGCCTTTATAGGGA |
| TUR | 57,122,337 | 57,122,356 | CCAGCCTGGAGCTGCTATTC |
| TDF | 57,152,045 | 57,152,069 | GGTAGTAAGAGTATGGAGTCAGTCC |
| TDP | 57,152,079 | 57,152,108 | CAAGCGTTTGAGGGCTACTAATGCTCTGTA |
| TDR | 57,152,138 | 57,152,159 | TCCTGGTTCCTAAGCCTGTATG |
PCR Primers
| Primer | Sequence |
| SU | ATTCTAGGCAACCGCCTGC |
| SD | GCATGGCCAATTACTGTTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 115 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15823A-E7 | VGB6 | C57BL/6NTac | |
| 15823B-B12 | VGB6 | C57BL/6NTac | |
| 15823B-A9 | VGB6 | C57BL/6NTac | |
| 15823C-C8 | VGB6 | C57BL/6NTac | |
