| Gene | Gabpa |
| Chromosome | 16 |
| Deletion Start | 84,860,655 |
| Deletion End | 84,839,110 |
| Deletion Size | 21,546 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 84,840,019 | 84,840,039 | CGAAGGAACAGTGGTGAGATG |
| TUP | 84,840,042 | 84,840,068 | AGCTACCATACCTGACTTTCCTTGGGC |
| TUR | 84,840,075 | 84,840,094 | TGAGGCCCAGAGTTCAGATG |
| TDF | 84,860,459 | 84,860,478 | TGGATACAGTGCAGCAGAAC |
| TDP | 84,860,479 | 84,860,506 | TGAACCGTCTGGTCATAGAGTGTGAACA |
| TDR | 84,860,510 | 84,860,527 | TGCATCCGTGCCAGTTTC |
PCR Primers
| Primer | Sequence |
| SU | CCAACAAGTTCCAATCCATAG |
| SD | ATCTTCAGACGCTACTGGCT |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15828A-C6 | VGB6 | C57BL/6NTac | |
| 15828A-C12 | VGB6 | C57BL/6NTac | |
| 15828A-E4 | VGB6 | C57BL/6NTac | |
| 15828A-E5 | VGB6 | C57BL/6NTac | |
| 15828A-G8 | VGB6 | C57BL/6NTac | |
