| Gene | Gart |
| Chromosome | 16 |
| Deletion Start | 91,643,098 |
| Deletion End | 91,621,532 |
| Deletion Size | 21,567 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 91,642,162 | 91,642,182 | CCTTCCTGACTCGACCAGAAC |
| TUP | 91,642,137 | 91,642,160 | AGCCTCCCAGTTTGCCTTAGAGCA |
| TUR | 91,642,105 | 91,642,127 | GCCATAGCAAATACGGACAAAGC |
| TDF | 91,622,120 | 91,622,138 | TGCCAGCCCTGTTCTCTTC |
| TDP | 91,622,093 | 91,622,118 | TTAAGACTCTGACTGCAGCCTGGTGT |
| TDR | 91,622,067 | 91,622,084 | GCCCACGAATGTGCAGAC |
PCR Primers
| Primer | Sequence |
| SU | CAAATGATTATGTGAAAATCCTAT |
| SD | AAGGCTGCAGTTTAAGGATCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15829A-A5 | VGB6 | C57BL/6NTac | |
| 15829A-B5 | VGB6 | C57BL/6NTac | |
| 15829A-E6 | VGB6 | C57BL/6NTac | |
| 15829A-E10 | VGB6 | C57BL/6NTac | |
| 15829A-H9 | VGB6 | C57BL/6NTac | |
