| Gene | 4930539E08Rik |
| Chromosome | 17 |
| Deletion Start | 28,915,230 |
| Deletion End | 28,898,852 |
| Deletion Size | 16,379 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 28,915,154 | 28,915,177 | TGGTCTGAGGTAGGTTGAATCTTC |
| TUP | 28,915,120 | 28,915,145 | AACCAGGGCAATGGGTGGGTATTTCA |
| TUR | 28,915,071 | 28,915,094 | GGTCTGGATTCGGGTCTATTACTC |
| TDF | 28,899,199 | 28,899,222 | TGGGACACAAAGACAAGAGCTAAG |
| TDP | 28,899,171 | 28,899,197 | TGTAGTCAGCTTCAAAGGACAGGCAAC |
| TDR | 28,899,145 | 28,899,162 | GGGACCAACTCGCAACAC |
PCR Primers
| Primer | Sequence |
| SU | GTTTCTAAGCGCTGGTTTCG |
| SD | CTCCACCCAGTGACACACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 107 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15842A-D2 | VGB6 | C57BL/6NTac | |
| 15842A-D7 | VGB6 | C57BL/6NTac | |
| 15842A-D9 | VGB6 | C57BL/6NTac | |
| 15842A-G11 | VGB6 | C57BL/6NTac | |
| 15842A-E7 | VGB6 | C57BL/6NTac | |
| 15842A-E10 | VGB6 | C57BL/6NTac | |
