| Gene | Abcg1 |
| Chromosome | 17 |
| Deletion Start | 31,064,623 |
| Deletion End | 31,057,818 |
| Deletion Size | 6,806 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 31,057,820 | 31,057,840 | CTGTCTGATGGCCGCTTTCTC |
| TUP | 31,057,843 | 31,057,862 | TCGGCACCGCCATGGTGAGT |
| TUR | 31,057,904 | 31,057,925 | CTGGCTTTGAGCTTCCTGTTTC |
| TDF | 31,063,770 | 31,063,789 | GTTGCCAGTGCCTCAGTTTC |
| TDP | 31,063,790 | 31,063,812 | CCTGTGCCTCAGTTTCCCTGCCT |
| TDR | 31,063,832 | 31,063,851 | CGGAGGATCAGGGTTGTTTG |
PCR Primers
| Primer | Sequence |
| SU | TTCGTCTAGGGTTGAGCTGG |
| SD | CGAAGTTTAGAGCCTCTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15844A-E1 | VGB6 | C57BL/6NTac | |
| 15844C-E1 | VGB6 | C57BL/6NTac | |
