| Gene | Atf6b |
| Chromosome | 17 |
| Deletion Start | 34,654,795 |
| Deletion End | 34,647,238 |
| Deletion Size | 7,558 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,648,107 | 34,648,130 | GTAAACTATGGAGCCTTGACACAG |
| TUP | 34,648,131 | 34,648,157 | TGGGCGTCTTAATTTGCAAGCTGTGTC |
| TUR | 34,648,178 | 34,648,197 | CGGGCAGGAAGGTGAGAAAG |
| TDF | 34,654,179 | 34,654,200 | GGAGTCTGAGATGGGAAGATGG |
| TDP | 34,654,203 | 34,654,230 | AGTCTGGGTTGCACAGTGAGATCCTTTC |
| TDR | 34,654,232 | 34,654,251 | GAGCAGCTTTGGTCTGGTTG |
PCR Primers
| Primer | Sequence |
| SU | CCTAAGTGCTAGGAACTACAT |
| SD | ATGCTGGCCCTATACACGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15847A-A1 | VGB6 | C57BL/6NTac | |
| 15847A-C3 | VGB6 | C57BL/6NTac | |
| 15847A-H11 | VGB6 | C57BL/6NTac | |
| 15847A-E11 | VGB6 | C57BL/6NTac | |
| 15847A-H8 | VGB6 | C57BL/6NTac | |
| 15847A-C11 | VGB6 | C57BL/6NTac | |
