| Gene | Rsph9 |
| Chromosome | 17 |
| Deletion Start | 46,144,145 |
| Deletion End | 46,129,334 |
| Deletion Size | 14,812 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 46,143,604 | 46,143,624 | AGCGGGCACAGAATGTAGAAG |
| TUP | 46,143,581 | 46,143,603 | TCCCGGAGATGGCAACTGGTGTA |
| TUR | 46,143,550 | 46,143,572 | CAAATCCAGTCCACAGCTAGAAC |
| TDF | 46,129,604 | 46,129,625 | GTCCCTGAAGTCACCTCTACAC |
| TDP | 46,129,579 | 46,129,602 | TGCTCCCTTCCCTGTGTCATGAGC |
| TDR | 46,129,544 | 46,129,565 | GAAAGTTGCTGTGCTCCTGATG |
PCR Primers
| Primer | Sequence |
| SU | GAGCCAAGGTATCAGATTC |
| SD | TGTGCATAGTAGGGAACTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15851A-F9 | VGB6 | C57BL/6NTac | |
| 15851A-C9 | VGB6 | C57BL/6NTac | |
