| Gene | Slc22a7 |
| Chromosome | 17 |
| Deletion Start | 46,438,386 |
| Deletion End | 46,432,403 |
| Deletion Size | 5,984 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 46,438,360 | 46,438,379 | AAGAGCTGCTGCACAAGGTG |
| TUP | 46,438,335 | 46,438,357 | TGGCTTTGGGCCTTTCCAACTGC |
| TUR | 46,438,289 | 46,438,307 | TGCATCGGGAGCAGGAATC |
| TDF | 46,433,006 | 46,433,024 | TGCCTGCACTGTACTCCTG |
| TDP | 46,432,981 | 46,433,004 | TGCCTGAGACGAAGAAAGCACAGC |
| TDR | 46,432,958 | 46,432,977 | CACGTCCTGGATGGTCTCTG |
PCR Primers
| Primer | Sequence |
| SU | AGGCCTCACTCAATCCACAC |
| SD | CATAGCTCTGTGCTTTAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15853A-B2 | VGB6 | C57BL/6NTac | |
| 15853A-G9 | VGB6 | C57BL/6NTac | |
