| Gene | Pex6 |
| Chromosome | 17 |
| Deletion Start | 46,725,398 |
| Deletion End | 46,711,503 |
| Deletion Size | 13,896 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 46,712,036 | 46,712,055 | GCCCGTAGTGTCGTCCTTTG |
| TUP | 46,712,059 | 46,712,080 | CTTCCCACTCGGTCCGGCGACT |
| TUR | 46,712,088 | 46,712,107 | TCTCCAGTCCCTCCCAGAAC |
| TDF | 46,724,765 | 46,724,785 | GCATGACTTCTGAGGGAGGAG |
| TDP | 46,724,787 | 46,724,811 | TGAGGCCAGCTTTAACATCCCTGGT |
| TDR | 46,724,815 | 46,724,836 | TCCAGCTTGAACCTATCACACC |
PCR Primers
| Primer | Sequence |
| SU | CACCGATTACTCTTGGTC |
| SD | CAGTCTGCATTCTCCAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15854A-B7 | VGB6 | C57BL/6NTac | |
| 15854A-G4 | VGB6 | C57BL/6NTac | |
