| Gene | Msh2 |
| Chromosome | 17 |
| Deletion Start | 87,696,598 |
| Deletion End | 87,672,599 |
| Deletion Size | 24,000 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 87,673,019 | 87,673,039 | TCCAGCCTCCCAAGTTGATAG |
| TUP | 87,673,044 | 87,673,068 | AGAGTCTTGTGTCACCGTGGGTACC |
| TUR | 87,673,071 | 87,673,092 | GCTGCGGGCTGTTATTGATTTG |
| TDF | 87,696,002 | 87,696,021 | ACAGGGCTGTGCTGAGGATG |
| TDP | 87,696,022 | 87,696,045 | AGAAAGCCACAGAGCAGCATGCCC |
| TDR | 87,696,071 | 87,696,094 | CAGGAACCAACAATGTGCAAGAAG |
PCR Primers
| Primer | Sequence |
| SU | CTGCAAGGCTGCAGAAGTC |
| SD | TAGCCAGGCATTCCACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15866A-D9 | VGB6 | C57BL/6NTac | |
| 15866A-B1 | VGB6 | C57BL/6NTac | |
