| Gene | Syt4 |
| Chromosome | 18 |
| Deletion Start | 31,447,164 |
| Deletion End | 31,440,107 |
| Deletion Size | 7,058 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 31,446,936 | 31,446,957 | GCCTTGTGATTTCACCTTCCAG |
| TUP | 31,446,911 | 31,446,934 | AGTTCCCGAATGTTCACGCGGCTC |
| TUR | 31,446,870 | 31,446,893 | CAGATGTCCTTTTCTTTCCTCAGC |
| TDF | 31,440,785 | 31,440,808 | CTTACCAAGATCACAAAGCGAGTG |
| TDP | 31,440,756 | 31,440,784 | AGCCCAGAAAGGGTGAGATTTACCTTCCC |
| TDR | 31,440,693 | 31,440,713 | TGCCGTCAGGGAAGATAAGAG |
PCR Primers
| Primer | Sequence |
| SU | GCTGATTTCAGCCCTGTAGC |
| SD | CCCAACCTGAAGAAACATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 116 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15873A-C5 | VGB6 | C57BL/6NTac | |
| 15873A-C8 | VGB6 | C57BL/6NTac | |
