| Gene | Rell2 |
| Chromosome | 18 |
| Deletion Start | 37,958,479 |
| Deletion End | 37,956,387 |
| Deletion Size | 2,093 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 37,956,440 | 37,956,458 | CCTGCTTGTGCTGGTCTTC |
| TUP | 37,956,460 | 37,956,483 | TCCTCATGGGCCTTGTAGGCTTCA |
| TUR | 37,956,503 | 37,956,521 | GCAGCGGTAGCCTTTCTTC |
| TDF | 37,957,900 | 37,957,918 | GGCACAGTATCCCACAGTC |
| TDP | 37,957,920 | 37,957,944 | CAGAAGGCTTCCCTACCCAAGGTCA |
| TDR | 37,957,960 | 37,957,980 | ACAGCTCAGAGGCTCCTAAAC |
PCR Primers
| Primer | Sequence |
| SU | ACTCAAGCGAGATGTCTG |
| SD | CAGAGCTGTCCAGTGGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15880A-C2 | VGB6 | C57BL/6NTac | |
| 15880A-D9 | VGB6 | C57BL/6NTac | |
| 15880A-F1 | VGB6 | C57BL/6NTac | |
| 15880A-E11 | VGB6 | C57BL/6NTac | |
| 15880A-H7 | VGB6 | C57BL/6NTac | |
