| Gene | Pcdh1 |
| Chromosome | 18 |
| Deletion Start | 38,203,577 |
| Deletion End | 38,197,097 |
| Deletion Size | 6,481 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 38,203,167 | 38,203,187 | GGCCAGATAGAGGTGCAAGAC |
| TUP | 38,203,140 | 38,203,164 | CAATGACAACACGCCGAACTTTGCC |
| TUR | 38,203,092 | 38,203,110 | CGAGCCGATGTTGGTGTTC |
| TDF | 38,197,695 | 38,197,716 | GCTAAGGGAAGCAAGAGCAAAG |
| TDP | 38,197,659 | 38,197,688 | AAAGCAAGTCACCGAAACCTGTGAAGCCAG |
| TDR | 38,197,633 | 38,197,653 | CAGCCCGGTGTCATCTTCATC |
PCR Primers
| Primer | Sequence |
| SU | CCAGGGAACGTCAGCTGG |
| SD | CCCAGATTCTAGGTCTTCCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15881A-D1 | VGB6 | C57BL/6NTac | |
| 15881A-F6 | VGB6 | C57BL/6NTac | |
| 15881A-F11 | VGB6 | C57BL/6NTac | |
| 15881A-G12 | VGB6 | C57BL/6NTac | |
| 15881A-H2 | VGB6 | C57BL/6NTac | |
