15882 Grxcr2 Status: ES cell colonies screened / QC positives
| Gene | Grxcr2 |
| Chromosome | 18 |
| Deletion Start | 41,998,999 |
| Deletion End | 41,986,410 |
| Deletion Size | 12,590 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 41,998,850 | 41,998,873 | TCGCCCAAAGAAGAATATCCTCAC |
| TUP | 41,998,822 | 41,998,846 | TTTCTCCAGGAGGCCCTTGAACCAA |
| TUR | 41,998,799 | 41,998,819 | CCCAGACCCATAAACGCCATC |
| TDF | 41,986,899 | 41,986,922 | CAGAAAGATGCAGAATGCCAGATG |
| TDP | 41,986,873 | 41,986,898 | TGACGGCAGATGTTGTACTCATCGCA |
| TDR | 41,986,826 | 41,986,846 | AACGGCCAGGACACAGAACAG |
PCR Primers
| Primer | Sequence |
| SU | AATTGATCAAATGCCCAACC |
| SD | AACAGGAATCTGGTTTGTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
