| Gene | Rbm22 |
| Chromosome | 18 |
| Deletion Start | 60,571,972 |
| Deletion End | 60,560,829 |
| Deletion Size | 11,144 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 60,561,156 | 60,561,174 | TGCCACCCTTGCATCCTTC |
| TUP | 60,561,187 | 60,561,213 | AAAGTGACCGAGGGAACACGGTCTTCG |
| TUR | 60,561,263 | 60,561,283 | TCCAAGACACGTCTGACACAG |
| TDF | 60,571,632 | 60,571,652 | AACAGGTGCATGAGTGTGGAG |
| TDP | 60,571,660 | 60,571,687 | ATCGAGGGAAAGACAAGGAATGAAGGCA |
| TDR | 60,571,688 | 60,571,711 | GTACAAGACTTGCTGAGGACTATC |
PCR Primers
| Primer | Sequence |
| SU | GTGGAAACAAGATAAAGGATGTC |
| SD | GCCACTACAATGATTAGTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 128 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15888A-D4 | VGB6 | C57BL/6NTac | |
| 15888A-C12 | VGB6 | C57BL/6NTac | |
| 15888A-C1 | VGB6 | C57BL/6NTac | |
