| Gene | Napg |
| Chromosome | 18 |
| Deletion Start | 62,997,037 |
| Deletion End | 62,977,972 |
| Deletion Size | 19,066 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 62,978,745 | 62,978,766 | CCCTTTCTGGTGTACCACCTTG |
| TUP | 62,978,770 | 62,978,794 | CCTGGCAGTGGCTAGAAAGATGGCT |
| TUR | 62,978,797 | 62,978,816 | TGGGTTCACCCTCTGCATTG |
| TDF | 62,996,560 | 62,996,583 | GTGTTACGACTGTTTGCCTGAATG |
| TDP | 62,996,586 | 62,996,610 | TGTGCACCACATGGAAGTCAGAGGA |
| TDR | 62,996,631 | 62,996,652 | GACTCACAACCTCCTGAACTCC |
PCR Primers
| Primer | Sequence |
| SU | TGTAAAGTGCAGTCGCCAAC |
| SD | TCGAAAGAACCAGACCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15889A-B2 | VGB6 | C57BL/6NTac | |
| 15889A-B6 | VGB6 | C57BL/6NTac | |
| 15889A-D5 | VGB6 | C57BL/6NTac | |
| 15889A-E1 | VGB6 | C57BL/6NTac | |
| 15889A-E11 | VGB6 | C57BL/6NTac | |
