| Gene | Pmaip1 |
| Chromosome | 18 |
| Deletion Start | 66,463,370 |
| Deletion End | 66,458,657 |
| Deletion Size | 4,714 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 66,458,941 | 66,458,959 | GGCTCGTGACTTCGACTGG |
| TUP | 66,458,969 | 66,458,996 | TTCTTCAGGGCTTCTACCGAGACTGTGG |
| TUR | 66,459,007 | 66,459,026 | TGATACGCTGGACTGGACTC |
| TDF | 66,463,146 | 66,463,165 | GCGCAGGAAAGTCACAGAAG |
| TDP | 66,463,175 | 66,463,203 | TGTGCCTTTCACTTGGGCTGTGTATTAAC |
| TDR | 66,463,219 | 66,463,238 | GGACAACCCTGAGTCTCAAC |
PCR Primers
| Primer | Sequence |
| SU | GAAGTTTCCCTCCCACCTTC |
| SD | AAAGCAATCCCAAACGACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15891A-C5 | VGB6 | C57BL/6NTac | |
| 15891A-A3 | VGB6 | C57BL/6NTac | |
