| Gene | Mrpl21 |
| Chromosome | 19 |
| Deletion Start | 3,289,083 |
| Deletion End | 3,283,075 |
| Deletion Size | 6,009 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 3,283,289 | 3,283,311 | CACAGGGTTTGGAGTACGAGAAC |
| TUP | 3,283,313 | 3,283,337 | TGGGATGAGAACGTGGTTCCTTCCG |
| TUR | 3,283,412 | 3,283,431 | CCCTGGGTAGGTCATTTGTC |
| TDF | 3,288,853 | 3,288,870 | TGGTCTGGGCTGCATGAG |
| TDP | 3,288,873 | 3,288,899 | CCCACTGCAGAACCAGGAATTCTCTGG |
| TDR | 3,288,938 | 3,288,957 | TGTGACAGTGCTCCTCCTTC |
PCR Primers
| Primer | Sequence |
| SU | CAAACTTACCTGCGCTCCTC |
| SD | CCTGACTTGTGCAGAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 143 bp | + | + | - |
| TDF | TDR | 105 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15898A-C8 | VGB6 | C57BL/6NTac | |
| 15898A-C9 | VGB6 | C57BL/6NTac | |
| 15898A-C11 | VGB6 | C57BL/6NTac | |
| 15898A-E3 | VGB6 | C57BL/6NTac | |
| 15898A-D10 | VGB6 | C57BL/6NTac | |
| 15898A-D11 | VGB6 | C57BL/6NTac | |
