| Gene | Yif1a |
| Chromosome | 19 |
| Deletion Start | 5,092,501 |
| Deletion End | 5,088,721 |
| Deletion Size | 3,781 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 5,089,017 | 5,089,037 | CTTACAACTCGGGCAGCATTC |
| TUP | 5,089,039 | 5,089,063 | TGACCACTGTCCAGGGACTCCGAAT |
| TUR | 5,089,104 | 5,089,123 | GGCACAATCACCGCAAAGAC |
| TDF | 5,091,582 | 5,091,601 | TGGTGTGGGTGTTCATGGAG |
| TDP | 5,091,603 | 5,091,626 | TGTTGGCTCTGCTTCTGGGCCTCT |
| TDR | 5,091,665 | 5,091,685 | GTAACCGCTGTAAGCCAAGAG |
PCR Primers
| Primer | Sequence |
| SU | GACGCGGAACTAAGTTAC |
| SD | TGCTTCTGGAAGGCTTGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 107 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15899A-B6 | VGB6 | C57BL/6NTac | |
| 15899A-D2 | VGB6 | C57BL/6NTac | |
| 15899A-D4 | VGB6 | C57BL/6NTac | |
| 15899A-G2 | VGB6 | C57BL/6NTac | |
| 15899A-H7 | VGB6 | C57BL/6NTac | |
