| Gene | Naaladl1 |
| Chromosome | 19 |
| Deletion Start | 6,115,579 |
| Deletion End | 6,105,801 |
| Deletion Size | 9,779 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 6,106,595 | 6,106,619 | CTTGCCCAGGTTGTTTATTCCTATC |
| TUP | 6,106,621 | 6,106,644 | TCCATCAGTCATGGTGGTCCCTGC |
| TUR | 6,106,666 | 6,106,689 | GGTGGTTACCCTCTTCTCTATCTC |
| TDF | 6,114,898 | 6,114,918 | TCCTTGGGTGAGCACATCTTG |
| TDP | 6,114,920 | 6,114,942 | CCCTGCAGAAGAGCAGCCCTGAG |
| TDR | 6,114,944 | 6,114,962 | CTGCCCTCTGCTGACACTC |
PCR Primers
| Primer | Sequence |
| SU | ACAGTCAGGGACTGACCGAC |
| SD | TCATTTATTTGAGGGTTAAGAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 65 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15903A-B2 | VGB6 | C57BL/6NTac | |
| 15903A-C6 | VGB6 | C57BL/6NTac | |
| 15903A-H4 | VGB6 | C57BL/6NTac | |
| 15903A-G7 | VGB6 | C57BL/6NTac | |
| 15903A-G8 | VGB6 | C57BL/6NTac | |
| 15903A-F9 | VGB6 | C57BL/6NTac | |
