| Gene | Atl3 |
| Chromosome | 19 |
| Deletion Start | 7,534,044 |
| Deletion End | 7,494,619 |
| Deletion Size | 39,426 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 7,494,960 | 7,494,978 | TTGGCGGTCCGTCTTAACG |
| TUP | 7,494,986 | 7,495,012 | ATCGGCGGAAGGCTTTATTGTTGAGCC |
| TUR | 7,495,018 | 7,495,035 | ACCGCAGCATTCCAGCAC |
| TDF | 7,533,422 | 7,533,445 | GAACTCTTGATTCTGCTGCCTCTC |
| TDP | 7,533,448 | 7,533,475 | TGGCTTCTAAGTGCTGCAAATGCTGTGG |
| TDR | 7,533,495 | 7,533,514 | TGGAGCCCATCCTGGTGTAG |
PCR Primers
| Primer | Sequence |
| SU | TGTCCTCCGTTTCCTCTACG |
| SD | ATTTCATGAGGTTGCCCTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15904A-A9 | VGB6 | C57BL/6NTac | |
| 15904A-C7 | VGB6 | C57BL/6NTac | |
| 15904A-E3 | VGB6 | C57BL/6NTac | |
| 15904A-H5 | VGB6 | C57BL/6NTac | |
| 15904A-H6 | VGB6 | C57BL/6NTac | |
