| Gene | Tmem138 |
| Chromosome | 19 |
| Deletion Start | 10,575,364 |
| Deletion End | 10,571,141 |
| Deletion Size | 4,224 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 10,574,627 | 10,574,645 | TCACTGGCCCTTTCACATC |
| TUP | 10,574,598 | 10,574,626 | TTTAAAGAGCTACAAGTGGCTGGGCATGG |
| TUR | 10,574,554 | 10,574,572 | TCCTGCCTTGGCCTGTTAG |
| TDF | 10,571,517 | 10,571,538 | CAGAGACTAGGTGAGGATGGAG |
| TDP | 10,571,491 | 10,571,513 | CTTCAGGTGCCCTCACAGCCTGC |
| TDR | 10,571,454 | 10,571,471 | TGGGAAGCAGGACCACAG |
PCR Primers
| Primer | Sequence |
| SU | AGTTGGGATGGGTTTGGG |
| SD | CTTTGCACTACAGTCTGCGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15908A-A6 | VGB6 | C57BL/6NTac | |
| 15908A-C12 | VGB6 | C57BL/6NTac | |
| 15908A-G4 | VGB6 | C57BL/6NTac | |
| 15908A-H10 | VGB6 | C57BL/6NTac | |
| 15908A-F9 | VGB6 | C57BL/6NTac | |
| 15908A-D5 | VGB6 | C57BL/6NTac | |
