| Gene | Ms4a7 |
| Chromosome | 19 |
| Deletion Start | 11,333,350 |
| Deletion End | 11,321,453 |
| Deletion Size | 11,898 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 11,332,734 | 11,332,755 | CCTGCTGAGACTCAATTCCTAC |
| TUP | 11,332,706 | 11,332,730 | TTCCCACCTTCAGAAACCTTCCTGC |
| TUR | 11,332,646 | 11,332,664 | TGAGGCTTTGGCCTTTGTC |
| TDF | 11,322,397 | 11,322,417 | CCTGTGCTGCTTTGCAGAGTG |
| TDP | 11,322,360 | 11,322,387 | TGATGCTTCTCTTCACTGTGCTGGAGGT |
| TDR | 11,322,299 | 11,322,319 | CCCAGGTTTGTTGGAGTAGAC |
PCR Primers
| Primer | Sequence |
| SU | TGGATAGAGTGCAGTCTATTC |
| SD | ATTGTGCAAGCAATGCAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 119 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15910A-A2 | VGB6 | C57BL/6NTac | |
| 15910A-H11 | VGB6 | C57BL/6NTac | |
| 15910A-C5 | VGB6 | C57BL/6NTac | |
| 15910A-H10 | VGB6 | C57BL/6NTac | |
| 15910A-G7 | VGB6 | C57BL/6NTac | |
| 15910A-E2 | VGB6 | C57BL/6NTac | |
