| Gene | Actr1a |
| Chromosome | 19 |
| Deletion Start | 46,395,674 |
| Deletion End | 46,378,365 |
| Deletion Size | 17,310 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 46,395,289 | 46,395,309 | TGGCTACTCTTGCTGTCTATC |
| TUP | 46,395,264 | 46,395,288 | TTAGCCTATTGGTCCCGCCTTTGGG |
| TUR | 46,395,234 | 46,395,255 | ACTGCGGAGTGCTGTTCTAATC |
| TDF | 46,379,240 | 46,379,258 | CCATGCCCTCTCCAGACAG |
| TDP | 46,379,208 | 46,379,235 | CCTGTAATGCTTGCTCACCCTGTAATGC |
| TDR | 46,379,185 | 46,379,206 | GGCCACATGAAACTCATCACTG |
PCR Primers
| Primer | Sequence |
| SU | CGAAGGGATCTCAAATCCAG |
| SD | AGCACAGAAGGATGAGCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15927A-A10 | VGB6 | C57BL/6NTac | |
| 15927A-D11 | VGB6 | C57BL/6NTac | |
| 15927A-E8 | VGB6 | C57BL/6NTac | |
| 15927A-E9 | VGB6 | C57BL/6NTac | |
| 15927A-G5 | VGB6 | C57BL/6NTac | |
