| Gene | Atp1b1 |
| Chromosome | 1 |
| Deletion Start | 164,457,854 |
| Deletion End | 164,438,192 |
| Deletion Size | 19,663 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 164,457,700 | 164,457,719 | TTTCTTAGGAGGCGCGTCTG |
| TUP | 164,457,679 | 164,457,699 | TACGCGAGCACTGCGGACAGT |
| TUR | 164,457,642 | 164,457,663 | CACTCCCTAACACCCTGGTCAG |
| TDF | 164,438,387 | 164,438,405 | GCAGTTCACCAACCTCACC |
| TDP | 164,438,364 | 164,438,385 | TGGACACTGAAATCCGCGTCGA |
| TDR | 164,438,344 | 164,438,363 | TCTCACCATACGCCTTACAC |
PCR Primers
| Primer | Sequence |
| SU | CGGAAGGCGATCGGAGTG |
| SD | ACCTTGGACAGCAAACCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 62 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15959A-D5 | VGB6 | C57BL/6NTac | |
| 15959A-E7 | VGB6 | C57BL/6NTac | |
