| Gene | Mical1 |
| Chromosome | 10 |
| Deletion Start | 41,486,946 |
| Deletion End | 41,478,060 |
| Deletion Size | 8,887 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 41,478,077 | 41,478,095 | CAACCCAGCACATGACCAC |
| TUP | 41,478,098 | 41,478,120 | TGAGACCTTTGTGCAGGCACAGC |
| TUR | 41,478,157 | 41,478,175 | TCCACTCCCAGGGCTCTAC |
| TDF | 41,485,991 | 41,486,009 | GCAATCAATCCGGCGTGTC |
| TDP | 41,486,011 | 41,486,034 | TGCCTTACAACCCACAGGCCATCC |
| TDR | 41,486,060 | 41,486,078 | CTCCAGCTCCCGCATAGTG |
PCR Primers
| Primer | Sequence |
| SU | GCTCCCTTAGCATCCTTGTG |
| SD | ATGCTGCACGTTCTGACAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15964A-H11 | VGB6 | C57BL/6NTac | |
| 15964A-C7 | VGB6 | C57BL/6NTac | |
| 15964A-C10 | VGB6 | C57BL/6NTac | |
| 15964A-H5 | VGB6 | C57BL/6NTac | |
| 15964A-D6 | VGB6 | C57BL/6NTac | |
| 15964A-A1 | VGB6 | C57BL/6NTac | |
