| Gene | Izumo4 |
| Chromosome | 10 |
| Deletion Start | 80,705,224 |
| Deletion End | 80,702,719 |
| Deletion Size | 2,506 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 80,703,572 | 80,703,591 | TGAGGGAACGAGGCAAGAAG |
| TUP | 80,703,594 | 80,703,617 | TGCCACAAATCACAAGGCAGCCTA |
| TUR | 80,703,624 | 80,703,646 | CAGGATACCCTAGCACAGAGAAG |
| TDF | 80,704,648 | 80,704,665 | TGCCTGAACCATGCTGTG |
| TDP | 80,704,669 | 80,704,692 | CTTCCCTGCGGCCTCAACTTGGAT |
| TDR | 80,704,728 | 80,704,752 | CACCTGCTTCTTTGTAGCCTTATTC |
PCR Primers
| Primer | Sequence |
| SU | GGAGCCATCCGTGGGCAG |
| SD | TGGATAGGAGCGTAGGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 105 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15968A-H12 | VGB6 | C57BL/6NTac | |
| 15968A-A9 | VGB6 | C57BL/6NTac | |
| 15968A-B1 | VGB6 | C57BL/6NTac | |
| 15968A-C3 | VGB6 | C57BL/6NTac | |
| 15968A-C4 | VGB6 | C57BL/6NTac | |
| 15968A-E4 | VGB6 | C57BL/6NTac | |
