15969   Kera     Status: ES cell colonies screened / QC positives

GeneKera
Chromosome10
Deletion Start97,612,979
Deletion End97,608,784
Deletion Size4,196
Genome Build38
MGI IDMGI:1202398
NCBI Gene ID16545
BAC ID211I7
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 10

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF97,608,88897,608,910TGTCCACGACGACTTCTATTGTC
TUP97,608,91597,608,941AGAATGCTTCTGTCCTCCCAGTTTCCC
TUR97,608,96097,608,982GGATTTCTGTGAGACCTCTGTTC
TDF97,612,83697,612,853CCACACTGCGTGCAGAAC
TDP97,612,85897,612,883TGCCTTCATTCACGGACCACAACTGA
TDR97,612,90397,612,925GGATCGGTGGCTTGATTTCATTC

PCR Primers

PrimerSequence
SUCCCATCTCTGAGGAACGAAG
SDCTGCTAGCTCATTGTGGTGC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR95 bp++-
TDFTDR90 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
15969A-H12VGB6C57BL/6NTac
15969A-A9VGB6C57BL/6NTac
15969A-A12VGB6C57BL/6NTac
15969A-G12VGB6C57BL/6NTac
15969A-F12VGB6C57BL/6NTac
15969A-C11VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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