| Gene | Pawr |
| Chromosome | 10 |
| Deletion Start | 108,333,307 |
| Deletion End | 108,332,820 |
| Deletion Size | 488 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 108,332,846 | 108,332,864 | CAGCACCACCACGGACTTC |
| TUP | 108,332,869 | 108,332,890 | AGGAGTGGAAGGCGAAGCGCGA |
| TUR | 108,332,892 | 108,332,909 | CTGCTTGGCGCGCATCTT |
| TDF | 108,333,170 | 108,333,190 | CGATGAGGAGGAGCCGGATAG |
| TDP | 108,333,201 | 108,333,220 | AAGGGCCGCAGCTCGGGACC |
| TDR | 108,333,243 | 108,333,264 | GCAGCTTCCTCTTCTCGATCTG |
PCR Primers
| Primer | Sequence |
| SU | GGCCCATCTCTTCCTCTCTC |
| SD | GTCTAGGCTGCCAAATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 64 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15970A-B1 | VGB6 | C57BL/6NTac | |
| 15970A-C4 | VGB6 | C57BL/6NTac | |
| 15970A-C9 | VGB6 | C57BL/6NTac | |
| 15970A-D9 | VGB6 | C57BL/6NTac | |
| 15970A-D11 | VGB6 | C57BL/6NTac | |
