| Gene | Polr3a |
| Chromosome | 14 |
| Deletion Start | 24,486,913 |
| Deletion End | 24,449,035 |
| Deletion Size | 37,879 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | TGGACGGAACAGTGTCAGAAC |
| TUP | | | TTCTGGGCGAAGATGCCACCTCC |
| TUR | | | GAAGAGGTCCGCTGAGAGTAATC |
| TDF | | | GTGTAGGTTGGCGCTTTGTC |
| TDP | | | TGACATCAACCTGTAATGTGTCTGCCTC |
| TDR | | | GCTCTGTGCATGTCTCTGATTC |
PCR Primers
| Primer | Sequence |
| SU | ACGCCCGGCCTCTTCTGC |
| SD | TGGGTCCTAGTCTCTGTGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16008A-A1 | VGB6 | C57BL/6NTac | |
| 16008A-D11 | VGB6 | C57BL/6NTac | |
| 16008A-E6 | VGB6 | C57BL/6NTac | |
