| Gene | Krt1 |
| Chromosome | 15 |
| Deletion Start | 101,850,724 |
| Deletion End | 101,845,840 |
| Deletion Size | 4,885 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 101,849,912 | 101,849,930 | GGGAGCCTGACGATGAGTG |
| TUP | 101,849,886 | 101,849,911 | TGGCTGACATCCCACTGCATTATCAC |
| TUR | 101,849,850 | 101,849,871 | TCAACAGATGTGAGCCCGATTC |
| TDF | 101,845,932 | 101,845,953 | GGCAGTTCTACCGTGAAGTTTG |
| TDP | 101,845,906 | 101,845,929 | TCCACCAGTTACTCCCGAGGGACC |
| TDR | 101,845,860 | 101,845,882 | TTGATGGAAAGGCAGAGCTAATG |
PCR Primers
| Primer | Sequence |
| SU | TACCACACGAGCCATTCTTG |
| SD | AAAGACAAACTCGCAAACACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16027A-A6 | VGB6 | C57BL/6NTac | |
| 16027A-C11 | VGB6 | C57BL/6NTac | |
| 16027A-G1 | VGB6 | C57BL/6NTac | |
| 16027A-F4 | VGB6 | C57BL/6NTac | |
| 16027A-C10 | VGB6 | C57BL/6NTac | |
