| Gene | Morc1 |
| Chromosome | 16 |
| Deletion Start | 48,437,324 |
| Deletion End | 48,431,344 |
| Deletion Size | 5,981 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 48,431,853 | 48,431,874 | GCTGTCAGACTAGAGGCAAGAC |
| TUP | 48,431,876 | 48,431,901 | AGGCAGTTGGGAGAACCAGCTGCTCT |
| TUR | 48,431,945 | 48,431,968 | TGTTAGGAGTTACAGAGGGTTGTG |
| TDF | 48,436,689 | 48,436,709 | GGACAGAGGCAGGTAGAACTC |
| TDP | 48,436,710 | 48,436,733 | TGTGTTTGAGGCCAGCCCTGTCTA |
| TDR | 48,436,736 | 48,436,756 | TGTCCTGGAACTCATTCACTC |
PCR Primers
| Primer | Sequence |
| SU | AGGCCAAACCAGACACTTTC |
| SD | CTTGGATTGTATAGGAAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 116 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16030A-F4 | VGB6 | C57BL/6NTac | |
| 16030A-G6 | VGB6 | C57BL/6NTac | |
| 16030A-H11 | VGB6 | C57BL/6NTac | |
