| Gene | Nit2 |
| Chromosome | 16 |
| Deletion Start | 57,167,284 |
| Deletion End | 57,157,058 |
| Deletion Size | 10,227 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 57,167,028 | 57,167,045 | CCAACTCGTGGCTCTGAC |
| TUP | 57,167,001 | 57,167,025 | CCTCGCCTCACAAAGCGGTGGATAA |
| TUR | 57,166,944 | 57,166,966 | GCCAGGACAGTGCAACTATAAAC |
| TDF | 57,158,024 | 57,158,044 | GCTCCTGTCAGCACACTCTTG |
| TDP | 57,157,991 | 57,158,018 | CAGACACTGGGTAAAGTCTTGTCCTCCA |
| TDR | 57,157,959 | 57,157,979 | TCCCTGGCTCCCTATGCTTTG |
PCR Primers
| Primer | Sequence |
| SU | TCAGCATGGAGCATCGTTAC |
| SD | TTCTCAGACAGATGGGACCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16031A-H12 | VGB6 | C57BL/6NTac | |
| 16031A-B6 | VGB6 | C57BL/6NTac | |
