| Gene | Zfp52 |
| Chromosome | 17 |
| Deletion Start | 21,562,073 |
| Deletion End | 21,555,049 |
| Deletion Size | 7,025 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 21,555,574 | 21,555,598 | AAGCTGTTATTTGATGCAGAGAAAC |
| TUP | 21,555,603 | 21,555,632 | CCCTCATTATGGCCTTGCTCTAGACACTTT |
| TUR | 21,555,633 | 21,555,653 | AGGCATGGAGTGCTGACATTC |
| TDF | 21,561,184 | 21,561,206 | TCATGCTGGAGAGAGACGTTATC |
| TDP | 21,561,212 | 21,561,241 | AAGGAATGTGGCAGATCTTTTACCTGGTTC |
| TDR | 21,561,248 | 21,561,271 | ATGAACTCGCTGATGGACTCTAAG |
PCR Primers
| Primer | Sequence |
| SU | TTGTGATAATTCATGACTGTCCTC |
| SD | AGCTTTTCTCAGGGATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16039A-F12 | VGB6 | C57BL/6NTac | |
| 16039A-B5 | VGB6 | C57BL/6NTac | |
