| Gene | Kifc5b |
| Chromosome | 17 |
| Deletion Start | 26,932,153 |
| Deletion End | 26,917,271 |
| Deletion Size | 14,883 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 26,917,604 | 26,917,629 | GACATGTTTGTGCTTCTCAGTTCTTG |
| TUP | 26,917,630 | 26,917,653 | CAGATCGCCTTGTGGGAGAAGCGA |
| TUR | 26,917,663 | 26,917,682 | GACAAGCGACAAGGCTTCTC |
| TDF | 26,931,904 | 26,931,930 | CACTATAAAGCTTCGTCTTTTCAGTCC |
| TDP | 26,931,935 | 26,931,962 | TCTTCCAGGCTTAGTGGACATAGCATCA |
| TDR | 26,931,986 | 26,932,009 | AAGTCTCTGTTATGGAAGCAGGTC |
PCR Primers
| Primer | Sequence |
| SU | CACTTCTGACGCAACCAGAG |
| SD | ATACCTTCCTTTGGCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16041A-D5 | VGB6 | C57BL/6NTac | |
| 16041A-F7 | VGB6 | C57BL/6NTac | |
