| Gene | Prrc2a |
| Chromosome | 17 |
| Deletion Start | 35,162,980 |
| Deletion End | 35,149,174 |
| Deletion Size | 13,807 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 35,162,239 | 35,162,258 | AGTGCCGAAAGACGGAACAG |
| TUP | 35,162,213 | 35,162,236 | TGGGCAAGCAAACAGGAGCAGTCC |
| TUR | 35,162,178 | 35,162,198 | TCAGGAAGTCCCACAGCTATC |
| TDF | 35,149,601 | 35,149,622 | ACCGTCACCTTTCCTCCCTTAG |
| TDP | 35,149,572 | 35,149,599 | CCCTTCTCTGGTCTCAATTCCCGTCTCA |
| TDR | 35,149,527 | 35,149,545 | TGCGCTGAGTTCGGAATAC |
PCR Primers
| Primer | Sequence |
| SU | TGGCACAAAGGATAGATGGG |
| SD | GAAACCCTAAGACAGGCACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16044A-A6 | VGB6 | C57BL/6NTac | |
| 16044A-G8 | VGB6 | C57BL/6NTac | |
| 16044A-E7 | VGB6 | C57BL/6NTac | |
| 16044A-F5 | VGB6 | C57BL/6NTac | |
| 16044A-F2 | VGB6 | C57BL/6NTac | |
