| Gene | Taf8 |
| Chromosome | 17 |
| Deletion Start | 47,502,266 |
| Deletion End | 47,489,834 |
| Deletion Size | 12,433 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 47,501,596 | 47,501,617 | CGGACTTGCTGGTCTTTCAGAG |
| TUP | 47,501,568 | 47,501,593 | CCTGAGTTTGGTGGAGTGGCTCACAA |
| TUR | 47,501,543 | 47,501,563 | TCCGTGAAGCAGGAGTTACAG |
| TDF | 47,490,382 | 47,490,399 | GAAGGCGGTGCTCTTGTC |
| TDP | 47,490,352 | 47,490,381 | TCCTCATATCTCATTAGACACCAGAGTGGC |
| TDR | 47,490,318 | 47,490,335 | TGGCGTGAGGAAGGAGAC |
PCR Primers
| Primer | Sequence |
| SU | GAACTTACTTCATACAAATGGC |
| SD | GGGTCCAGGTGAGAAGTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16047B-G4 | VGB6 | C57BL/6NTac | |
| 16047C-C9 | VGB6 | C57BL/6NTac | |
| 16047C-E12 | VGB6 | C57BL/6NTac | |
| 16047C-H9 | VGB6 | C57BL/6NTac | |
