| Gene | Ralbp1 |
| Chromosome | 17 |
| Deletion Start | 65,867,772 |
| Deletion End | 65,864,278 |
| Deletion Size | 3,495 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 65,866,902 | 65,866,922 | GCTCACGTCTTTCTTCACAGG |
| TUP | 65,866,879 | 65,866,901 | TTGGCACTGCCTGAGTTGGAGCT |
| TUR | 65,866,837 | 65,866,859 | TCAACCCAACCAATCAACCAAAC |
| TDF | 65,864,380 | 65,864,400 | CCCTTGGTTGACGCAGTAGAG |
| TDP | 65,864,355 | 65,864,379 | AGGACCATGATGTACGACGGCGTCC |
| TDR | 65,864,309 | 65,864,330 | TGCTTCTCCATGTAGTCCACAC |
PCR Primers
| Primer | Sequence |
| SU | AGTGCGAGAGGGTCCTAGTG |
| SD | AAGACGCTGAGACAAGACTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16050A-F10 | VGB6 | C57BL/6NTac | |
| 16050A-A7 | VGB6 | C57BL/6NTac | |
| 16050A-B7 | VGB6 | C57BL/6NTac | |
