| Gene | Sos1 |
| Chromosome | 17 |
| Deletion Start | 80,479,920 |
| Deletion End | 80,449,263 |
| Deletion Size | 30,658 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 80,479,486 | 80,479,507 | GGTACTCTCACTGGCTCGAAAG |
| TUP | 80,479,465 | 80,479,485 | TGCCTTGGCCACAGTGCTTGC |
| TUR | 80,479,418 | 80,479,437 | CCGAGCAGGCCATGTTCTTG |
| TDF | 80,450,197 | 80,450,221 | TGTTTGCATAGAATGTTCCCAGTAG |
| TDP | 80,450,175 | 80,450,196 | TAGATGCGCACAGCAGGGCTCT |
| TDR | 80,450,140 | 80,450,162 | GCAAAAGTGACTTCCCATTGTTC |
PCR Primers
| Primer | Sequence |
| SU | CATCCACGCGCCGTCTCT |
| SD | GTGATCTGAGCAGTGAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16053A-A5 | VGB6 | C57BL/6NTac | |
| 16053A-C10 | VGB6 | C57BL/6NTac | |
| 16053A-E5 | VGB6 | C57BL/6NTac | |
| 16053A-F8 | VGB6 | C57BL/6NTac | |
| 16053A-G12 | VGB6 | C57BL/6NTac | |
| 16053A-H10 | VGB6 | C57BL/6NTac | |
