| Gene | Slc3a1 |
| Chromosome | 17 |
| Deletion Start | 85,064,109 |
| Deletion End | 85,028,435 |
| Deletion Size | 35,675 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 85,028,836 | 85,028,858 | GGATGGGAATGGAGACCTGAAAG |
| TUP | 85,028,862 | 85,028,883 | CGTGTTCGCCGCTCAGGACCAA |
| TUR | 85,028,908 | 85,028,927 | CACAAGCCAGGCACAGAATG |
| TDF | 85,063,200 | 85,063,222 | CAGCGTGCAGTGTTCTTTCTATC |
| TDP | 85,063,224 | 85,063,249 | TCAGCGATCTGAGAATCCGCATCTGC |
| TDR | 85,063,278 | 85,063,296 | CTTGGGCTTGGTCCTCATC |
PCR Primers
| Primer | Sequence |
| SU | CGCATTACTGTAGGCAGTTGG |
| SD | GAGAAGGGAGCCAGAAGGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16054A-A3 | VGB6 | C57BL/6NTac | |
| 16054A-G2 | VGB6 | C57BL/6NTac | |
| 16054A-D2 | VGB6 | C57BL/6NTac | |
