| Gene | Mep1b |
| Chromosome | 18 |
| Deletion Start | 21,100,122 |
| Deletion End | 21,072,377 |
| Deletion Size | 27,746 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 21,072,751 | 21,072,775 | TGACACAGGTGAAAACTGACAAATG |
| TUP | 21,072,776 | 21,072,800 | TCAGAGGCAAGCAATGTCCACATCA |
| TUR | 21,072,825 | 21,072,847 | AGACTGGCAAAGATGCAGAAGAG |
| TDF | 21,099,442 | 21,099,466 | TGTGAGTTTACGTGACTGTCAAGAG |
| TDP | 21,099,471 | 21,099,495 | TCCCTAGAAACTGAGCAGTGTGGGA |
| TDR | 21,099,499 | 21,099,520 | ACATGCACTGAGCCCTTACAAC |
PCR Primers
| Primer | Sequence |
| SU | CAGTGAGAGGTTGGTTGATCC |
| SD | CATTAAGGCAGGTTCAAGTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16055A-F12 | VGB6 | C57BL/6NTac | |
| 16055A-E2 | VGB6 | C57BL/6NTac | |
| 16055A-D4 | VGB6 | C57BL/6NTac | |
| 16055A-A5 | VGB6 | C57BL/6NTac | |
| 16055A-A9 | VGB6 | C57BL/6NTac | |
