| Gene | Tmco6 |
| Chromosome | 18 |
| Deletion Start | 36,742,254 |
| Deletion End | 36,735,175 |
| Deletion Size | 7,080 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,735,835 | 36,735,855 | CGTCAACCCAGCTACTGTCTC |
| TUP | 36,735,860 | 36,735,885 | TCAGTTCCCGGAACATAGACCAGCTG |
| TUR | 36,735,937 | 36,735,956 | GCAGGAGGATTGCCAGTTTG |
| TDF | 36,741,657 | 36,741,674 | TGCAGGTGCTCACAGTTC |
| TDP | 36,741,675 | 36,741,700 | TGTGCAATGTCGTAGAGAAGGGTCCA |
| TDR | 36,741,702 | 36,741,721 | CCACAACCGCTGGCAGTAAG |
PCR Primers
| Primer | Sequence |
| SU | AGTGCTCATCAGTCGAAGCC |
| SD | CAAAGCCTGAAGGTCTCCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 122 bp | + | + | - |
| TDF | TDR | 65 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16057B-F4 | VGB6 | C57BL/6NTac | |
| 16057B-G8 | VGB6 | C57BL/6NTac | |
