| Gene | Sncaip |
| Chromosome | 18 |
| Deletion Start | 52,871,477 |
| Deletion End | 52,838,013 |
| Deletion Size | 33,465 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 52,838,568 | 52,838,588 | GTACCCAGCAGTCATACCAAC |
| TUP | 52,838,591 | 52,838,615 | TGTAGAACTCTAGCTCCAGGGCAGC |
| TUR | 52,838,625 | 52,838,645 | GCAGTGTCTAAGGGAGGTCAG |
| TDF | 52,870,825 | 52,870,848 | AGGGTGGTGACAGAGGTAGAGAAG |
| TDP | 52,870,850 | 52,870,877 | TGTGGCTTATCATCAGTTTCACCTCGGG |
| TDR | 52,870,890 | 52,870,911 | CGAAGGTTTCCAGTTCCAGTTG |
PCR Primers
| Primer | Sequence |
| SU | TTGCAGGAGATGGTCTTTCC |
| SD | CTCCGAGAGAGCCAGGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16060A-E3 | VGB6 | C57BL/6NTac | |
| 16060B-G12 | VGB6 | C57BL/6NTac | |
