16061   Rnmt     Status: ES cell colonies screened / QC positives

GeneRnmt
Chromosome18
Deletion Start68,321,387
Deletion End68,305,825
Deletion Size15,563
Genome Build38
MGI IDMGI:1915147
NCBI Gene ID67897
BAC ID214J17
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 18

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF68,305,84668,305,865GTCGCTTCTGACCCAGAGTC
TUP68,305,86968,305,890TCCAGGTGGTAATGAGCCCGCT
TUR68,305,92268,305,941TACTTGCTGGCATGGAGGAG
TDF68,320,65268,320,675GCATTCTGCCATGAATCTACTTTC
TDP68,320,67768,320,703TCAGAGGAGAATGCATAGTCTGGCAGA
TDR68,320,70868,320,728CTGTGGAAGCCCAGGACTTTG

PCR Primers

PrimerSequence
SUGTTTGGAGGCATCCTAATTAATAC
SDCCTCTGCCAATTCTAGCGAC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR96 bp++-
TDFTDR77 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
16061A-F10VGB6C57BL/6NTac
16061B-D5VGB6C57BL/6NTac
16061B-A2VGB6C57BL/6NTac
16061C-F11VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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