| Gene | Pip5k1b |
| Chromosome | 19 |
| Deletion Start | 24,439,118 |
| Deletion End | 24,397,019 |
| Deletion Size | 42,100 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GTGCCGTTCTGCTGTTGTG |
| TUP | | | TGAGGACTTTAGACCTTTCCTTCCTGTGA |
| TUR | | | AGAGCCGCAAGAGAAAGTAAGAC |
| TDF | | | GGAGGCATACGCTGGAAC |
| TDP | | | TCTTCACCTACAGCTAGATAGGCACAGAAA |
| TDR | | | GCTGGTGACATAGGCCATTG |
PCR Primers
| Primer | Sequence |
| SU | TGGTACTTTCCAGATATCGT |
| SD | CCAGGCACCCACTACAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16063B-A3 | VGB6 | C57BL/6NTac | |
| 16063B-C6 | VGB6 | C57BL/6NTac | |
| 16063B-G4 | VGB6 | C57BL/6NTac | |
| 16063B-H7 | VGB6 | C57BL/6NTac | |
