| Gene | Lipn |
| Chromosome | 19 |
| Deletion Start | 34,084,870 |
| Deletion End | 34,068,632 |
| Deletion Size | 16,239 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,069,473 | 34,069,494 | TGGGTACATTCTCGCCATCAAC |
| TUP | 34,069,510 | 34,069,534 | AGAGCACAGACTGGGCAGACAGGTA |
| TUR | 34,069,586 | 34,069,607 | TGTCTCCCTTCCAATGAATAGC |
| TDF | 34,084,613 | 34,084,636 | CAGAGTTACCCTCCCTTGTATGAC |
| TDP | 34,084,638 | 34,084,660 | TGACGGCCATGAAGGTACCCACT |
| TDR | 34,084,663 | 34,084,684 | ATCGTGTCCACCAGCCCAAATG |
PCR Primers
| Primer | Sequence |
| SU | GGCCAGTGTGATGACTTG |
| SD | TCAAATCCATGGCTTCACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 135 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16064A-B12 | VGB6 | C57BL/6NTac | |
| 16064A-D7 | VGB6 | C57BL/6NTac | |
