| Gene | 5930434B04Rik |
| Chromosome | 2 |
| Deletion Start | 27,019,056 |
| Deletion End | 27,009,991 |
| Deletion Size | 9,066 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 27,010,430 | 27,010,447 | GGGCAGTGATCGGAGTTC |
| TUP | 27,010,448 | 27,010,471 | TCCTCGGGTTCCTACTGTCCACAC |
| TUR | 27,010,476 | 27,010,495 | AGGCCGACGAGAAGCATTAG |
| TDF | 27,018,103 | 27,018,125 | GGGATGTGGGTGTAGGATAAATG |
| TDP | 27,018,131 | 27,018,155 | CTGCCCTCACCTAGATGTCAGGACC |
| TDR | 27,018,189 | 27,018,207 | ACAGCCCTGTGACCAAGTG |
PCR Primers
| Primer | Sequence |
| SU | CTTGGAGGTCTGAACAAGGC |
| SD | TCTGACGACAGGTCATGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 105 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16068A-F5 | VGB6 | C57BL/6NTac | |
| 16068A-G3 | VGB6 | C57BL/6NTac | |
| 16068A-G9 | VGB6 | C57BL/6NTac | |
