| Gene | Tspan18 |
| Chromosome | 2 |
| Deletion Start | 93,222,771 |
| Deletion End | 93,204,330 |
| Deletion Size | 18,442 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 93,221,897 | 93,221,915 | TCGGAGGAGGCTGTCTTGG |
| TUP | 93,221,875 | 93,221,896 | CATCCCAGCTCTGCAGCCTGGA |
| TUR | 93,221,847 | 93,221,868 | GTTCAGGTTCAGAGTGGTGGAG |
| TDF | 93,204,430 | 93,204,452 | CTCCAGATCATCTTCTCCCTTTC |
| TDP | 93,204,402 | 93,204,427 | TGCAGCTCTTCCTCATGGTCTTTGCC |
| TDR | 93,204,345 | 93,204,364 | GTGGTGGGCTGAGTGTCTTC |
PCR Primers
| Primer | Sequence |
| SU | TAGGACTGGTCTGTCCTCGC |
| SD | TGCTGCAGAGTCTGTCCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16072A-C11 | VGB6 | C57BL/6NTac | |
| 16072A-D12 | VGB6 | C57BL/6NTac | |
| 16072A-E7 | VGB6 | C57BL/6NTac | |
| 16072A-G3 | VGB6 | C57BL/6NTac | |
| 16072A-E12 | VGB6 | C57BL/6NTac | |
