| Gene | Spef1 |
| Chromosome | 2 |
| Deletion Start | 131,174,670 |
| Deletion End | 131,171,584 |
| Deletion Size | 3,087 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 131,174,118 | 131,174,138 | TGTGGTGCTAGGGAGTCAAAC |
| TUP | 131,174,094 | 131,174,117 | CCAGGGCATTGTGAACGCTAAGCA |
| TUR | 131,174,068 | 131,174,091 | GGATTTAGCCCTGTTGGTAGCAAG |
| TDF | 131,171,819 | 131,171,838 | GCAAGGCAACAGGTCTTCAG |
| TDP | 131,171,793 | 131,171,815 | TGATGTCTCTTGCGTCGCCACCC |
| TDR | 131,171,763 | 131,171,782 | CCAGACGCTTCACCTTCATC |
PCR Primers
| Primer | Sequence |
| SU | AGGTGGTGTTGGCAGATAGG |
| SD | GGAGGCCTTGGAGATTTAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16074A-A3 | VGB6 | C57BL/6NTac | |
| 16074A-G11 | VGB6 | C57BL/6NTac | |
| 16074A-A12 | VGB6 | C57BL/6NTac | |
| 16074A-G8 | VGB6 | C57BL/6NTac | |
| 16074A-E3 | VGB6 | C57BL/6NTac | |
| 16074A-F10 | VGB6 | C57BL/6NTac | |
